BRD3-024 BrilliantDye™ Terminator v3.1 Cycle Sequencing Kit

BrilliantDye® Terminator Cycle Sequencing Kits are high-performance reagents designed for DNA sequencing using the well-established Sanger sequencing method. These kits serve as a direct drop-in alternative to BigDye™, offering excellent compatibility, flexibility, and reliable sequencing results.

They are fully compatible with all ABI Genetic Analyzers and require no changes to existing protocols, workflows, or instrument settings, making them an efficient and cost-effective solution for laboratories.

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Description

The BrilliantDye™ Terminator v3.1 Cycle Sequencing Kit provides ready-to-use reagents for Sanger sequencing, including BrilliantDye™ Terminator ready-reaction premix, 5x sequencing buffer, pGEM control, and M13 (-21) primer. Optimized for de-novo sequencing and resequencing applications, this kit offers robust and flexible chemistry for various DNA sequencing needs.

This kit ensures high-quality sequencing with uniform peak heights, optimized signal balance, and accurate base assignments. It serves as a direct replacement for BigDye™ Terminator v1.1 without requiring protocol adjustments. Compatible with PCR fragments, plasmids, and single- or double-stranded DNA templates, it integrates seamlessly into existing workflows.

Compatibility
•    Applied Biosystems™ 310, 3100, 3130, 3500, 3730 Series Genetic Analyzers
•    SeqStudio™ and SeqStudio™ Flex
•    Promega™ Spectrum Compact CE System

Kit Components
•    Ready Reaction (RR) Sequencing Premix
•    5x Sequencing Buffer
•    pGEM Control DNA
•    M13 (-21) Primer (Forward)

Availability
•    Available in multiple formats, from 24, 100, 1000, 5000, and 25.000 rxn

Benefits
✓ Available in multiple reaction formats
✓ High signal balance for long, accurate reads
✓ Ready-to-use reagents for Sanger sequencing
✓ Compatible with multiple sequencing platforms
✓ No protocol, volume, setting, or spectral calibration changes are needed

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