4995352 – 2xTaq PCR MasterMix II (with dye) 300ul

The 2× Taq PCR MasterMix II is a ready-to-use PCR premix containing Taq DNA polymerase, dNTPs, MgCl₂, reaction buffer, and PCR enhancers. It simplifies PCR setup, minimizes handling errors, and reduces contamination risk while delivering high sensitivity and specificity.

It performs reliably with GC-rich (>60%), complex, or impure templates and generates PCR products with a 3′-dA overhang, making them suitable for direct TA cloning after purification.

Description

The 2× Taq PCR MasterMix II is a ready-to-use PCR premix containing Taq DNA polymerase, dNTPs, MgCl₂, reaction buffer, and PCR enhancers. It simplifies PCR setup, minimizes handling errors, and reduces contamination risk while delivering high sensitivity and specificity.

It performs reliably with GC-rich (>60%), complex, or impure templates and generates PCR products with a 3′-dA overhang, making them suitable for direct TA cloning after purification.

Key Features

  • Ready-to-use 2× PCR premix

  • High amplification efficiency and sensitivity

  • Strong tolerance to impure or crude templates

  • Suitable for GC-rich and complex templates

  • PCR products compatible with TA cloning

Applications

  • Routine PCR amplification

  • Large-scale gene detection

  • Semi-quantitative PCR

  • Trace DNA detection

  • DNA labeling and sequencing preparation

Storage

  • –20 °C for long-term storage

  • 4 °C for frequent use

  • Stable through repeated freeze–thaw cycles

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