Description
The FastKing One Step RT-qPCR Kit (SYBR Green) enables reverse transcription and real-time PCR in a single tube, simplifying workflow and reducing the risk of contamination. It is optimized for SYBR Green I–based detection and delivers high sensitivity, specificity, and reproducibility across a wide dynamic range.
The kit combines FastKing Reverse Transcriptase, an antibody-modified hot-start Taq DNA polymerase, and RNase inhibitor in a single enzyme mix, ensuring efficient cDNA synthesis and accurate amplification—even for RNA templates with complex secondary structures.
Key Features
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One-step RT-qPCR in a single tube
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SYBR Green I–based real-time detection
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High sensitivity for low-abundance targets
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Excellent specificity with hot-start Taq polymerase
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Reliable and reproducible quantification
Applications
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Gene expression analysis
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Quantitative RNA detection
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Real-time RT-qPCR of low- and high-abundance targets
Storage
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Store at –30 °C to –15 °C, protected from light
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Stable for up to 1 year
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